Monocyte chemoattractant protein-1 in the dental pulp of the immunohistochemical study.doc

 PAGE \* MERGEFORMAT 11 Monocyte chemoattractant protein-1 in the dental pulp of the immunohistochemical study Paper Keywords monocyte chemoattractant protein-1 pulpitis Immunohistochemistry  Abstract Objective: To investigate monocyte chemoattractant protein-1 (MCP-1) in pulpitis occurrence and development mechanism of action, in order to reveal the MCP-1 Dui pulpitis disease occurrence and development provide the basis for the role. Methods: Immunohistochemical staining to detect normal and acute and chronic pulpitis pulp tissue in patients with MCP-1 expression. Results: MCP-1 in normal human dental pulp tissue no expression in inflammatory tissue have different levels of expression.  Materials and Methods  Test specimens from the Stomatology Hospital in Changchun City in 2004 3 to September Oral Medicine outpatient, acute and chronic pulpitis all 20 cases, 20 cases of healthy pulp (porcelain teeth need to pull pulp persons), patients aged 18 to 50 years old , all patients within 2 months without taking immunosuppressive drugs and anti-bacterial drug history, were not done endodontic therapy.  Specimen processing: samples soaked with 10% neutral formalin, routine dehydration, embedded in paraffin to prepare 4 ~ 5μm thick slices, respectively, for HE staining and immunohistochemical staining. HE staining results as pathological grouping criteria, to further differentiate specimens of each group.  Main reagents, instruments: MCP-1 monoclonal antibody (TBD company), SABC color kit (Wuhan Boster Company), SP hypersensitivity Kit (taking of new biological).  Immunohistochemical staining steps: hydration of paraffin sections of conventional dewaxing, PBS wash 3 minutes × 3 times, high-temperature heating repair antigen (heated to 95 ℃ off fire, natural cooling at room temperature, distilled water and washed three times, PBS soak 5 minutes) ; PBS wash three minutes × 3 times; dropping peroxidase blocking solution (Reag