Monoclonal antibody against -amyloid protein and the establishment of hybridoma immunological characteristics of.docVIP

 PAGE \* MERGEFORMAT 11 Monoclonal antibody against -amyloid protein and the establishment of hybridoma immunological characteristics of Of: Zhang Yan Zhao Wei Wenqing warehouse full of coke Juan Liu Jing FU Yao [Abstract] Objective To prepare the anti-1 42 amyloid protein (A1 42 monoclonal antibody, and its immunological characteristics were analyzed for the further study of Alzheimer disease (AD pathogenesis basis. Methods synthetic A1 42 coupling hemocyanin (KLH into immunized BALB / c mice with anti-A1 42 Preparation of monoclonal antibodies, and immunological characteristics of antibody. Results from 24 holes were screened positive for specificity and efficiency 6 high prices of the cell line, named B1 ~ 6, in which B2, B3, B4, B6 are IgG2a subtype, B1, B5 belongs to IgG2b subtype. measured relative affinity of B6 lt;B1, B4 lt;B2 lt;B3, B5. Conclusion successfully prepared a specific, high titer of anti-A1 42 monoclonal antibody hybridoma cell lines for the next lay the foundation for further research. [Keywords:] 1 42 amyloid, Alzheimer’s disease, monoclonal antibodies, immunological characteristics A large number of epidemiology, neuropathology and animal models of amyloid Dengjun prove ( amyloid, A protein in Alzheimer disease (AD pathogenesis and plays a key role in the development of (1 ~ 3). So Many treatment methods targeted AD reduced deposition of A production and A clearance. Schnek, etc. (4) synthetic A1 42 polypeptide PDAPP mice, an animal model of more than 10 months of age appeared to be the basic phenomenon of senile plaque control. Janus etc. (5) in transgenic mice were carried out A vaccination experiments, A not only animals to alleviate the overload and the appearance of senile plaques was significantly reduced, and the vaccine was observed in animal models of cognitive dysfunction has a preventive effect. For this reason, Many scholars are committed to the development of different A fragments for polyclonal and monoclonal a