Mouse IL12 gene transfection of human ovarian cancer cell growth and cell Skov3 factor expression.doc

 PAGE \* MERGEFORMAT 6 Mouse IL12 gene transfection of human ovarian cancer cell growth and cell Skov3 factor expression [Keywords:] gene transfection Effect of mouse IL12 gene transfection on the growth of ovarian cancer Skov3 cells and the expression of related factors [Abstract] AIM: To investigate the effect on the proliferation of ovarian cancer Skov3 cells and the expression of related factors, by the plasmid with mouse IL12 gene transfected into Skov3 cells, which cultured with spleen cells in vitro. METHODS: Human ovarian cancer Skov3 cells were transfected with mouse IL12 gene by lipofect. The expressions of IL12 and INFγ were detected by ELISA, and the effects on the proliferation of Skov3 ovarian cancer cells were evaluated by MTT. RESULTS: A high expression level of IL12 (473 ± 38) ng / L was found 48 h after transfection, more higher than that in control group (13-17 ng / L, P amp;lt;0.05). Ovarian cancer cells could produce INFγ under the influence of spleen cells, and the highest level (173 ± 18) ng / L emerged 24 h after transfection, which also was significantly different from untransfected group (P amp;lt;0.05). Skov3 ovarian cancer cells proliferation was restrained by IL12 when they were cultured with spleen cells (P amp;lt;0.05). Immunochemistry revealed that the cancer cells could produce IL12 after transfection, and the latter could restrain the proliferation of Skov3 ovarian cancer cells. CONCLUSION: The IL12 genes could be transfected into Skov3 ovarian cancer cells to product IL12 and relative cell factors, which could restrain the proliferation of the cells and then kill the carcinoma cells of ovary. [Keywords] interleukin12; ovarian neoplasms; genetherapy [Abstract] Objective: To observe the full-length gene containing mouse IL12 plasmid was transfected into Skov3 human ovarian cancer cells, in the presence of spleen cells on tumor cells and related cytokines. Methods: liposome - dyeing technology to Skov3 gene transfer in h