Mouse MMP9 RNA interference lentiviral vector in vitro silencing.doc

 PAGE \* MERGEFORMAT 11 Mouse MMP9 RNA interference lentiviral vector in vitro silencing Abstract Objective To construct the mouse matrix metalloproteinase (MMP) 9 RNA interference (RNAi) lentiviral vector cells in vitro tools to silence effects. Methods for MMP 9 gene in mice constructed the expression of MMP 9 clones had plasmids and RNAi lentiviral vector co-transfected cells to develop good tools (293T and NIH3T3 cells), using real time quantitative PCR and Western blot observe the tool RNAi cells, MMP 9 mRNA and protein expression of downward effect. Was constructed of MMP 9 RNAi lentiviral vector could significantly reduce the tools cells the expression of MMP 9. Conclusion We successfully constructed to inhibit the expression of MMP 9 mice RNAi lentiviral vector for in vivo basis. Keywords: MMP 9; RNA interference; lentiviral vector Abstract Objective To explore the silencing effect of the constructed mice’s matrix metalloproteinase (MMP) 9 RNA interference (RNAi) lentivirus vector in vitro. Methods 293T and NIH3T3 cells were cocultured by MMP 9 overexpressed clone plasmid and RNAi lentivirus vector . Real time PCR and Western blot were adopted to observe the downregulation of MMP 9 mRNA expression in 293T and NIH3T3 cells by RNAi. Results The constructed MMP 9 RNAi lentivirus vector significantly decreased MMP 9 expression in 293T and NIH3T3 cells. Conclusions RNAi lentivirus vector inhibiting MMP 9 expression is successfully constructed, laying foundation for the research in vivo. Keywords: Matrix metalloproteinase (MMP) 9; RNA interference; Lentivirus vector Atherosclerosis (AS) plaque rupture is the main reason for acute ischemic events. Mechanisms of apoptosis and matrix degradation and plaque rupture related to the process 1. Cycle of the extracellular matrix (ECM) markers with the AS level is often closely related to disease risk stratification. Matrix metalloproteinases (MMPs) degrade ECM, undermine the integr