Mouse kidney development in neuronal nitric oxide synthase in quantitative analysis of.doc

 PAGE \* MERGEFORMAT 4 Mouse kidney development in neuronal nitric oxide synthase in quantitative analysis of Author: Liu Xia Xiao-ming MU march package Cuifen [Abstract] Objective: To postnatal mice at different stages of kidney development in neuronal nitric oxide synthase (nNOS) expression of quantitative analysis of nNOS in the mouse kidney development in meaning. Methods: Neonatal (birth less than 2h), days after birth 3,5,7,14,40 Kunming mice were all 8, a total of six groups, using Western blot techniques and optical density analysis of each group of mice after birth Renal neuronal nitric oxide synthase quantitative tests to the measured maximum amount of nitric oxide synthase 1 (100%), calculate the relative content of protein, SPSS10. 0 Statistical Package for statistical analysis. Results: The new group of up to 100% enzyme concentration, enzyme content decreased with age to adult minimum level of 44.8 ± 2.4%. Conclusion: This trend indicates that nNOS in mice after birth may be the early stages of kidney development plays an important regulatory role. [Keywords:] mice; kidney; neuronal nitric oxide synthase Of nitric oxide (NO) as an important messenger molecule in vivo and effects of molecules in the regulation of renal hemodynamics has an important role. The kidney, NO mainly by a nitric oxide synthase (NOS) catalytic produce. There are three subtypes of NOS, namely neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) [1]. The current occurrence of renal NO and NOS during the development of research still in its infancy, this experiment was detected by quantitative occurrence of postnatal mice at different stages of development of the content of nNOS, nNOS in the mouse to explore the significance of kidney development. 1 Materials and methods 1.1 Experimental animals and grouping Adult healthy Kunming mice (Experimental Animal Center of Liaoning Medical College). Under