Mouse IFN.doc

 PAGE \* MERGEFORMAT 11 Mouse IFN Author: surmise Xia, Gao Zhi-yun, FANG Wen-Liang, Jin-huai, ZHANG Yong-hong, Xie Lixin, WANG Yong-xiang [Keywords:] Interferon type Ⅱ ; intestinal virus B, human; vaccines, DNA; adjuvants, immunization; neutralizing antibody; mice Effect of mouse IFNγ gene adjuvant on immunogenic enhancement of secreting coxsackievirus B3 VP1 DNA vaccine [Abstract] AIM: To construct the eukaryotic expression plasmid pcDNA3/mIFNγ and observe its effect on secreting coxsackievirus B3 (CVB3) VP1 gene vaccine. METHODS: mIFNγ cDNA amplified by RTPCR was inserted in eukaryotic expression vector pcDNA3 and then detected after being transfected into cos7 cells with DEAEDextran; Balb / c mice were inoculated in quadriceps muscle at 2week interval for 6 weeks with pcDNA3/sVP1, pcDNA3/sVP1 pcDNA3/mIFNγ , respectively. The levels of serum antibodies were detected 13 d after each inoculation.The mice were challenged by 500 TCID50 CVB3 2 weeks after the last immunization. RESULTS: Recombinant pcDNA3/mIFNγ plasmid had been constructed and transcripted and expressed. The neutralizing antibody could be induced by pcDNA3/sVP1 and pcDNA3/sVP1 pcDNA3/mIFNγ ; The antibody titers increased with the times of inoculation. But there was no difference in the antibody titers and the survival rate between the 2 groups. Coadministration of pcDNA3/mIFNγ and pcDNA3/sVP1 plasmid to Balb / c mice, decreased the titers of virus and reduced the histopathological changes compared with control groups. CONCLUSION: mIFNγ can enhance the immune protection of secreting CVB3 DNA vaccine. [Keywords] interferon type Ⅱ ; enterovirus B, human; vaccines, DNA; adjuvants, immunologic; neutralizing antibody; mice [Abstract] Objective: To construct eukaryotic expression plasmid IFNγ in mice to observe their secretory Coxsackie virus B3 (CVB3) nucleic acid vaccine. Methods: The RTPCR amplification of mouse IFNγ gene eukaryotic expression plasmid pcDNA3 / mIFNγ , was detected in the