Neuro2A choroid plexus cells on the embryonic spinal motor axons repulsion.doc

 PAGE \* MERGEFORMAT 14 Neuro2A choroid plexus cells on the embryonic spinal motor axons repulsion [Abstract] Objective: To investigate the choroid plexus tissue, Neuro 2A cells on the embryonic spinal cord motor neurons axon growth. Methods: ECM Gel matrix as tissue culture substrates, the choroid plexus tissue or Neuro 2A cells and embryos 12 days of the ventral spinal cord motor column and three-dimensional co-culture, observe the choroid plexus tissue or Neuro 2A cells on the embryonic spinal cord motor neurons axon growth. Results: The choroid plexus tissue or Neuro 2A cells and embryos of 12 days ventral spinal cord tissue were cultured for 2 days, the performance of spinal motor axons to deviate from the choroid plexus tissue or cell line Neuro 2A biased growth. Conclusion: The choroid plexus tissue and Neuro 2A cells on the embryonic spinal motor axons a rejection. [Keywords:] Slit2 choroid plexus axons of spinal motor neurons were cultured Victoria [Abstract] Objective: To investigate the effect of the choroid plexus or the Neuro 2A cells on the embryonic spinal motor axons.Methods: To use ECM Gel as a tissue culture substrate, 3 dimention co culture of the choroids plexus or the Neuro 2A cells with the ventral part of the embryonic spinal motor column (Embryonic 12 days, E12) were made in the substrate.To observe the effect of the choroid plexus or the Neuro 2A cells on the embryonic spinal motor axons. Results: After 2 days of co culture, we found more spinal motor axons were grown from the distal part of the choroid plexus or the Neuro 2A cells. Conclusion: The choroid plexus and the Neuro 2A cells had the repelling effect on embryonic spinal motor axons. [Keywords:] Slit2; choroid plexus; the embryonic spinal motor axons; 3 dimention co culture Neuronal migration and axon guidance of the nervous system during development and regeneration of important issues. Currently found a series of neural migration and axon guidance related molec