Neurological function in different EAE in the mouse model to evaluate the standard of comparison.doc

 PAGE \* MERGEFORMAT 15 Neurological function in different EAE in the mouse model to evaluate the standard of comparison Of: Tongqiao Wen, Zhu Ying standard, Yaosu Qin, Li clank, summer reading frame, Han Zhao, Zhengrong far [Abstract] Objective: To compare the different neurological deficit scoring criteria on experimental autoimmune encephalomyelitis (EAE evaluate the performance of motor dysfunction. Methods: MOG35-55 peptide emulsified with complete Freund’s adjuvant to immunize 20 only the C57BL / 6 mice, replication EAE mouse model, using 3 different scoring criteria (Kono’s 5 points method, Hooper’s 7 points method, Weaver’s 15 points were observed and assessed the incidence of experimental animals, the experimental mice the early stages, the peak of neurological damage assessment of the quantitative features. Results: The 3 scoring comparison, in the early stages of disease, method and 7 minutes and 15 minutes for Evaluating the sensitivity of the symptoms of nerve injury rather than the method of high 5 points, peak, the symptom score and pathological score in level 3 score method to compare the validity of Act 15 the highest validity, followed by 7 points method. Conclusion: Weaver’s 15 points Evaluation of symptoms of EAE model of neurological injury, has obvious advantages, the study recommended as the preferred future EAE score. [Keywords:] experimental autoimmune encephalomyelitis model scoring Abstract: Objective: To compare several methods of clinical assessment standards in mice model of EAE. Methods: The mice models of experimental autoimmune encephalomyelitis (EAE) were established, and the neural symptoms and pathological changes were observed. Three clinical assessment methods, including 5 -point ,7-point and 15-point, were used to assess neurological function. Results: On the early onset ,7-point, the 15-point disease score scales were more sensitivity than 5-point score scale to the incidence of disease . And the 15-point disease