Neonatal mouse cochlea Corti in vitro and observation devices.doc

 PAGE \* MERGEFORMAT 9 Neonatal mouse cochlea Corti in vitro and observation devices Of: Xu Tao, Meng Xiuqing, Wangai Mei, Liu bimonthly, Mu Hua [Abstract] Objective To establish a neonatal mice in vitro cochlear Corti easy way to control and observe the cochlear hair cells of the tissue morphology. Methods 3 ~ 5 d after birth, mice cochlea, tile in the collagen gel surface, and then with 1% bovine serum albumin were cultured in DMEM/F12 medium. using tetramethyl rhodamine isothiocyanate labeled phalloidin staining to observe the growth of cochlear hair cells. Results of cochlea 24,48 h and 72 h after culture, the inner and outer hair cells were growing well, and its structural integrity, ciliary bundles arranged in order, without any loss. Conclusion The training method is simple and effective device for cochlear Corti in vitro experimental study . [Keywords:] mice; cochlea; vitro; Corti device Abstract: Objective To establish an simple method for the culture of the organ of Corti from newborn Kunming mouse, and to observe the histological morphology of cochlear hair cells. Methods The cochleae from Kunming mice of postnatal day 3 to 5 were used. The basilar membrane of the mouse was isolated and positioned on the surface of collagen gel, and then cultured in DMEM / F12 medium containing 1% bovine serum albumin. Tetramethylrhodamine isothiocyanate-labeled phalloidine staining was used to observe growing status of hair cells. Results Following culture of the cochlear basilar membrane for 24 hours, 48 #8203;#8203;hours and 72 hours, the inner and outer hair cells were both growing well. Their structures were intact, and stereociliary bundles were arranged orderly, without any deletion. Conclusions This method is simple and effective, which can be applied to the experimental study of the organ of Corti in vitro culture. Keywords:: mouse; cochlea; culture; organ of Corti Corti cochlear device is carried out in vitro inner ear physiology and patho