Neurotrophic factor family and its receptor in recombinant human bone morphogenetic protein.doc

 PAGE \* MERGEFORMAT 21 Neurotrophic factor family and its receptor in recombinant human bone morphogenetic protein Keywords: bone morphogenetic protein Abstract: [Objective] by observing the recombinant human bone morphogenetic protein-2 (rhBMP2) during osteoinduction neurotrophic factor (NTFs) and its receptor family, investigate the expression of neurotrophic factors in the role of BMP-induced bone. [Methods] To establish a mouse heterotopic the rear of the right femoral bone model, the experimental group implanted rhBMP2 collagen complex in the control group only the same volume of collagen sponge implant. , Respectively, after 7,14 and 21 d subjects, for histology, immunohistochemistry and RTPCR detection. [Results] with histologically confirmed rhBMP2 collagen-based composite has a good bone induction ability, while the control group, no bone. After the first 7 d, a large number of cartilage formation in the period of NGF and TrkA-positive staining reached a peak fibroblasts into cartilage cells, cartilage cells, hypertrophic chondrocytes and osteoblasts were positive for expression; BDNF in the cartilage cells and cartilage matrix There are positive staining, its receptor TrkB only in into cartilage cells and cartilage cells to express; NT3 and NGF expression is similar to its receptor TrkC only in cartilage cells and cartilage cells with positive staining. After the first 14 d, NGF and TrkA expression confined to parts of osteoblasts, bone-like cells and osteoblast-like cells; NT3 in the cartilage cells, osteogenic cells significantly expressed. After 21 d, only a small number of osteoblasts, osteoblast-like cells with NGF and TrkA expression. RTPCR results showed that, NTFs mRNA expression at 7 d after the first highest, and immunohistochemical expression of proteins consistent with the results. [Conclusion] NTFs and their receptors in bone formation process is obviously rhBMP2 induced expression, suggesting that NTFs may be through direct an