Nephritis on high performance liquid chromatography HPLC antipyretic tablets Forsythia.doc

 PAGE \* MERGEFORMAT 7 Nephritis on high performance liquid chromatography HPLC antipyretic tablets Forsythia Of: Xiao-Ming Fang, Cao Aimin, including permanent Rui [Abstract] Objective To develop nephritis phillyrin fever determination of the film. Methods using high performance liquid chromatography to shim-pak clc-ODS #8203;#8203;(4.6 mm × 150 mm, 5 μm) column using acetonitrile -0.025 mol / L phosphoric acid (20:80) as mobile phase, flow rate 1 mL / min, detection wavelength 277 nm. forsythin sample volume results in the range of 0.12 ~ 0.5 μg good linear relationship, the average recovery was 97.1 % (n = 5), RSD = 1.61%. Conclusion This determination method can be used as quality control of the preparation. [Keywords:] nephritis antipyretic tablets, phillyrin, HPLC, determination Abstract: Objective To establish a method for the determination of Forsythin in Shenyan Jiere Tablets by HPLC. Methods The separation was performed on shim-pak clc-ODS #8203;#8203;(4.6 mm × 150 mm, 5 μm) with Accetonitrile-0.025 mol / L H3PO4 ( 20:80) as mobile phase. The flow rate was 1 mL / min and UV detector was at 277 nm. Results The method had good average recovery with 97.1% (n = 5), RSD = 1.6%, good linear relationship within the range of 0.12 ~ 0.5 μg. Conclusion The method is accurate, reliable and can be used for quality control of the production. Keywords:: Shenyan Jiere Tablets, Forsythin, HPLC, content determination Nephritis by the Rhizoma Imperatae antipyretic tablets, forsythia composed of 14 herbs, with ease air heat, ventilating lung and Water features, for acute pneumonia TG cold light, the treatment of head, face and eyelid edema embolism. This test uses high-performance liquid chromatography (HPLC) method for the preparation of Forsythia Forsythia be measured anthocyanin content, the study methodology, methods, specificity, precision, reproducibility, recovery tests are in line with relevant provisions of the preparations for the establi