NF-κ B inhibitor pyrrolidine dithiocarbamate on human pterygium fibroblasts proliferation.doc

 PAGE \* MERGEFORMAT 2 NF-κ B inhibitor pyrrolidine dithiocarbamate on human pterygium fibroblasts proliferation [Abstract] Objective: To study the (nuclear factor, NF) 2κ B inhibitor pyrrolidine dithiocarbamate (NF-B Inhibitor pyrrolidine dithiocarbamate, PDTC) in cultured human pterygium fibroblasts (human pterygium fibroblast, HPF) proliferation the impact of adjuvant therapy and to find new ways to prevent recurrence of pterygium. Methods: human pterygium specimens for routine adherent cells cultured in vitro, take the first 3 to 6 on behalf of the cells tested. (1) immunohistochemical staining method combining cell biology characterization fibroblast; (2) different concentrations of PDTC (2.5,5,10,20,40 μ mol / L) interfere with fibroblast 24,48,72 h later, MTT method Results: In vitro cultured pterygium cells can be seen by immunohistochemical staining positive for vimentin expression, combined with cell biology to determine the characteristics of fibroblasts. MTT test showed that with the increase of PDTC in a concentration and time-extended fibroblast growth inhibition rate increased (P