Nickel sulfate-induced ovarian damage in female mice.doc

 PAGE \* MERGEFORMAT 5 Nickel sulfate-induced ovarian damage in female mice [Abstract] Objective To study the nickel sulphate-induced apoptosis in mouse ovarian toxicity and its mechanism for the prevention and treatment of premature ovarian failure caused by nickel toxicity provide a scientific basis. Methods Healthy female mice of Kunming species to each one 10  0,5  0,2  5mg/kg nickel sulfate (NiSO4) intraperitoneal injection of exposure, 0  2ml / (10g * bw), 1 times / d, continuous 12d. Estrous cycle, vaginal cytology was observed. Mouse ovary removal, preparation of ovarian patch observed morphological changes of ovary cells and ovarian cells using flow cytometry cell cycle and DNA content. Results (1) exposed mice diestrus to extend the irregular. (2) The optical microscope can be observed morphological changes of apoptosis. (3) The cell cycle analysis showed that with increasing dose of the mouse ovary cells showed G0/G1 phase cells were arrested and appeared before the Ap peak G1 peak (apoptotic peak, sub-diploid peak), G2 / M, S phase cell ratio decreased; apoptosis rate increased; cell proliferation index (PI) reduced; aneuploid index (DI) increased. Conclusion intraperitoneal injection of nickel sulfate can cause ovarian damage female mice, its role and NiSO4 damage and induction of cell cycle regulation of ovarian cell apoptosis. [Keywords:] nickel sulfate Nickel (Ni) is a trace element essential to the human condition, and one of common industrial and environmental contaminants, their toxicity can affect the liver, kidneys, lungs and cardiovascular and other systems, many of the body enzyme systems also has certain toxic effects 〔 1〕 . IARC (IARC) to nickel and its compounds classified as carcinogens in the first category. Previous studies confirmed that nickel compounds on the reproductive function of male and female sexes have adverse effects on the nickel interfere with the estrous cycle in mice, induced apoptosis in ovarian cells, c