Night incense tree extract on murine leukemia L1210 cell growth inhibition.doc

 PAGE \* MERGEFORMAT 9 Night incense tree extract on murine leukemia L1210 cell growth inhibition Author: Luo Jun Zhong Zhenguo Zhaohua Ping Zhao Yuan [Abstract] Objective To explore the different nights japonicum extracts L1210 murine leukemia cell lines and mechanism of inhibition of cell proliferation. Methods tetrazolium salt colorimetric (MTT) method, flat colony method and fluorescent staining Hoechst33258 night japonicum extract on L1210 cell growth inhibition and apoptosis. Results Night incense tree extract Chiang Kai-shek butanol extract and polysaccharide extract on L1210 cells in a concentration-dependent cytotoxicity, in the final concentration of 62.5 μ g * ml-1 range, the n-butanol extract and polysaccharide extract on L1210 Cell inhibitory rate of more than 90%, IC50 less than 10 μ g * ml-1, night fragrant tree is alcohol and polysaccharide extracts can inhibit the L1210 cells, colony formation and induce apoptosis in L1210 cells. Conclusion night incense tree extract on L1210 murine leukemia cell lines could inhibit cell proliferation by inducing apoptosis of L1210 cells play a role. [Keywords:] Night incense tree extract; murine leukemia cells; cell proliferation; colony-forming Abstract: ObjectiveTo evaluate the inhibitory effect of different extracts from Cestrum nocturnum Linn .. (CN) on mouse leukcmic L1210 cells and explore its mechanism. MethodsThe MTT assay, colony forming experiment and Hoechst 33258 fluorescence measurement were used to analyze the effect of extracts from CN on leukcmic L1210 cells. ResultsThe n-bulty alcohol part and polysaccharide part extracted from CN had dose-dependent effects of cytotoxicity.When the concentration was 62.5 μ g * ml-1, the inhibitory rate of cells growth was above 90% and the IC50 was below 10 μ g * ml-1.The inhibitory rate of cells had remarkable dose-dependence. They both could inhibit colony information and induce apoptosis of L1210 cells. ConclusionThe inhibitory effect of extracts fr