NMR signals in human urine internal standard method of quantitative analysis.doc

 PAGE \* MERGEFORMAT 20 NMR signals in human urine internal standard method of quantitative analysis Of: Yang Liang, Ru Court English, Tang Huiru Liu Chaoyang [Abstract] studied the electrical signal Varian NMR spectrometer internal standard method in the determination of metabolite concentrations in human urine on the application, the experiment proved that the reliability of quantitative analysis method. NMR signal is the standard method by the principle Varian spectrometer decoupling one-dimensional spectra in the regular channels to generate a reference signal, and using spectrometer software program to adjust the signal intensity in the spectrum, frequency, decay rate and other parameters. to avoid the quantitative metabolomics experiments in the NMR need to add a known concentration of substances (such as the internal standard TSP caused by the interaction with the sample internal standard, peak overlap, internal standard, such as insoluble problems and relaxation time is too long. The results show that, Varian spectrometer decoupling channel stable and reliable electrical signals generated (standard deviation of 0.36%, can be used for quantitative analysis, when the sample concentration is greater than 20 mmol / L or less than 2 mmol / L, the method and the relative error of 1% and 5%. through the preparation of low concentrations of urine model samples to verify the measurement of electrical signals within the standard concentration of metabolites in human urine feasibility, and finally the real use of the methods to measure metabolites in human urine concentrations of commonly measured results and the hospital Biochemical analysis of commonly used instruments testing results. [Keywords:] nuclear magnetic resonance; signals internal standard; human urine; quantitative analysis Abstract We report a complete assessment for urinary quantitative analysis by using an electronic signal as an internal reference, which was generated via decoupling c