Notch signaling pathway gene expression and adenoid cystic carcinoma metastasis.doc

 PAGE \* MERGEFORMAT 14 Notch signaling pathway gene expression and adenoid cystic carcinoma metastasis Of: Lu Youguang She Zhang Sheng Lin Lin Li, Song Ding Lincan [Abstract] Objective To screen and transfer of salivary adenoid cystic carcinoma of the Notch signaling pathway related genes. Methods that have been built high and low metastasis adenoid cystic carcinoma cell line ACC M, ACC 2 bioinformatics analysis of gene expression , with the Notch signaling pathway genes were compared, the differences in selected genes by fluorescent real-time quantitative PCR, differential expression of genes related to the initial validation. adenoid cystic carcinoma of the results of the expression profile of 46 genes in Notch signaling pathway. verify that, Notch 1, Notch 2, Notch 4, CHUK, FOXC1, FZD2, FZD3, GBP2, and RUNX1 in the two cell lines was significantly different expression (P lt;0.05), while Notch 1 In the event of transfer of salivary adenoid cystic carcinoma was significantly higher than that of metastatic salivary adenoid cystic carcinoma (P lt;0.05. Conclusion Notch signaling pathway may be involved in multiple genes in salivary gland adenoid cystic the occurrence and development of cancer, Notch 1 expression and adenoid cystic carcinoma and metastasis. [Keywords:] carcinoma, adenoid cystic, salivary gland tumors, polymerase chain reaction, cell culture, gene expression, signal transduction Adenoid cystic carcinoma is one of common malignant tumors in oral salivary gland, the high degree of malignancy of the tumor, postoperative easy to relapse, patients with poor prognosis. Now that the various elements involved in the progression and metastasis of tumors, including P53 , P16, P27, MMPs other. Notch signaling pathway is an ancient pathway, adjacent cells to pass through the Notch signaling pathway to determine cell proliferation or apoptosis, is involved in embryonic development, T cell development, and maintenance of hematopoietic stem cells se