Norepinephrine on pancreatic cancer cell line MiaPaCa2 invasion of.doc

 PAGE \* MERGEFORMAT 13 Norepinephrine on pancreatic cancer cell line MiaPaCa2 invasion of Of: Guo Kun, Chen Huan Sha, Ma Ching Chung, WANG Lian before, Hu Hengtong [Abstract] Objective To investigate the classical neurotransmitter norepinephrine (norepinephrine, NE) on human pancreatic cancer cell line MiaPaCa 2 invasion of its mechanism. Methods of human pancreatic cancer cell line MiaPaCa 2 for the study were divided blank control group, 0.1mol / L NE intervention group, 1mol / L NE intervention group and 10mol / L NE intervention group. intervention 48h, using Transwell invasion assay in MiaPaCa 2 in each group changes in cell invasion; RT PCR method Detection of cells MMP 2, MMP 9, and VEGF mRNA expression; cells were detected by immunocytochemistry in the MMP 2, MMP 9 and VEGF protein expression. Results 1mol / L NE intervention group and 10mol / L NE MiaPaCa 2 cells in the intervention group invasion were higher than the control group (P lt;0.01); 1mol / L NE intervention group and 10mol / L NE intervention group MiaPaCa 2 cells MMP 2, MMP 9, and VEGF mRNA and protein expression were higher than the control group ( P lt;0.05). Conclusion NE can increase MMP 2, MMP 9, and the expression of VEGF in pancreatic cancer cells to distant metastasis. [Keywords:] pancreatic cancer; cell invasion; neurotransmitter; norepinephrine ABSTRACT: Objective To investigate the effect of norepinephrine (NE) on the invasiveness of pancreatic cancer cell line MiaPaCa 2 and its action mechanism. Methods The pancreatic cancer cell line MiaPaCa 2 was used in the research. The cells were randomly divided into control group, 0.1mol / L NE intervention group, 1mol / L NE intervention group and 10mol / L NE intervention group. After intervention for 48h, Transwell invasiveness test was given to examine the changes of invasive ability of MiaPaCa 2. The expressions of MMP 2 mRNA, MMP 9 mRNA and VEGF mRNA were measured by semi quantitative RT PCR; the levels of MMP 2, MMP 9