Nucleic acid amplification technology in the diagnosis of tuberculosis and progress of.doc

 PAGE \* MERGEFORMAT 17 Nucleic acid amplification technology in the diagnosis of tuberculosis and progress of Over the past 10 odd years the rapid development of modern molecular biology for clinical laboratory diagnostics to provide a more reliable nucleic acid detection methods. Classic polymerase chain reaction (PCR) amplification technology has been widely used in the diagnosis of tuberculosis; However, due to the serious pollution problems, the presence of PCR inhibitors, kits lack of standardization, standardization, combined with technology, conditions, quality control and other factors influence the sensitivity and specificity varies widely, it is their credibility and reliability are suspect, to a clinician diagnosis of tuberculosis has brought a certain amount of confusion. To this end, foreign scholars to develop a high sensitivity, specificity, standardization and commercialization of PCR amplification kit; Following the PCR, the have developed several new in vitro nucleic acid amplification. Is now on its diagnosis of tuberculosis and progress of the application to make a presentation for the majority of fellow reference. 1, PCR-based amplification reaction that Roche Amplicor Mycobacterium tuberculosis PCR test (Amplicor pCR) Amplicor PCR is located in the Department of Application of biotin-labeled 16S rRNA gene of Mycobacterium tuberculosis highly conserved region of species-specific primers amplified Mycobacterium tuberculosis complex DNA in 584 bp sequence; biotin-labeled amplicon (amplicon) and species-specific DNA probe hybridization, the application of the standard colorimetric ELISA techniques identified, resulting in 450 nm wavelengths to determine the absorbance (A450)gt; 0.350 for the positive standard [1 ~ 3]. Amplicor pCR was developed by the Swiss company Roche standardization of commercial kits, the kit has three different small boxes: specimen boxes, amplification and identification of box box. In order