O-amino phenolDemethylepipodophyllotoxin toxins SOSP9607 anti-osteosarcoma effects and mechanisms of cell.docVIP

 PAGE \* MERGEFORMAT 10 O-amino phenolDemethylepipodophyllotoxin toxins SOSP9607 anti-osteosarcoma effects and mechanisms of cell [Abstract] Objective: To study the o-amino phenol  Demethylepipodophyllotoxin toxin (ODE) anti-osteosarcoma SOSP  9607 cells, the effect and mechanism. Methods: The MTT colorimetric assay in vitro anti-osteosarcoma SOSP  9607 cells, the role of transmission electron microscope morphology of apoptosis; measured using laser scanning confocal microscope SOSP  9607 cell [Ca2], [Mg2], pH value and the mitochondrial membrane potential (MMP) fluorescence intensity; immunocytochemistry to detect cells Bc1  2 , and Bax expression. Results: ODE time, concentration-dependent manner inhibited SOSP  9607 cell growth, induce apoptosis with typical morphological features of apoptosis such as the disappearance of cell surface microvilli, nuclear pyknosis, nuclear fragmentation, etc.; dose-dependent manner SOSP  9607 increase in intracellular Ca2, Mg2 lowered the concentration of intracellular pH, and mitochondrial membrane potential; so SOSP  9607 cells decreased expression of Bcl  2 and Bax protein expression increased. Conclusion: The o-amino phenol to a table  gui acetabular toxin in vitro could inhibit osteosarcoma SOSP  9607 cell growth, and its mechanism may be related to changes in the intracellular environment, steady-state and Bcl  2 and Bax protein expression and induced cell apoptosis. [Keywords:] o  aminophenol Demethylepipodophyllotoxin toxin osteosarcoma tumor cell apoptosis mitochondrial membrane potential of bcl  2 protein, Bax protein, 0 Introduction Osteosarcoma is a bone tissue originated from the most common malignant tumor, also known as osteosarcoma, any age, will be the disease, but young people aged 15 to 25 majority. Is a primary malignant bone tumor of the most common malignant highest degree of bone tumors, with early onset of distant metastasis (lung metastasis-prone) and the characteristic