Obese children in Baotou City of ApoE gene polymorphism.docVIP

 PAGE \* MERGEFORMAT 7 Obese children in Baotou City of ApoE gene polymorphism OF: Zhang Lifang Cheng Ziying Hui-Qin Wang Xing Ji less [Abstract] Objective: To study the Baotou City ApoE gene polymorphism in obese children. METHODS: Genomic white blood cells DNA, primer design ApoE gene, using polymerase chain reaction - Restriction fragment length polymorphism (PCR-RFLP ) technical analysis of its polymorphism. Results: In this study, ApoE 4 genotypes were detected, respectively, E3 / 3, E2 / 3, E3 / 4, E2 / 4, were not detected E2 / 2, E4 / 4 type ; obesity group and the control group alleles 2, 3, 4 frequency distribution uneven, 3 highest frequency; compared with healthy children, obese children increased 4 allele frequency, the difference was statistically significant (P lt;0.05 ). Conclusion: ApoE gene polymorphism and childhood obesity to some extent. [Keywords:] obese children; ApoE gene; polymorphism Ren Lei Apolipoprotein E (ApoE) gene located on chromosome 19, genetic polymorphism has obvious. ApoE You 3 main heterogeneous body, that is E2, E3, E4, respectively by a gene locus 3 alleles 2, 3, 4 coding. ApoE is one of the major apolipoprotein of serum, involved in lipid metabolism and regulation of body cholesterol balance [1]. ApoE isoforms and receptors of three different affinity of lipid metabolism to varying degrees, affect lipid levels. high blood cholesterol can cause atherosclerosis, hypertension, coronary heart disease, obesity and other chronic diseases, with potentially dangerous. In this paper, the city part of the ApoE gene polymorphism in obese children for the next step of ApoE gene polymorphism in obese children and blood relations with the basic materials research. 1 Materials and methods 1.1 Reagents Hha enzyme (U.S. BioLabs company), pBR322DNA/MspI marker (Beijing TIANGEN BIOTECH company), PCR amplification kit (Takara Company). 1.2 study According to lt;”China BMI young school-age children overweight an