Obese diabetic mouse pancreaticcell high-resolution 3-D imaging research.doc

 PAGE \* MERGEFORMAT 7 Obese diabetic mouse pancreaticcell high-resolution 3-D imaging research [Abstract] Objective: To study obesity, diabetes mice (ob / ob islet cells in vivo flavoprotein (Fp), reduced coenzyme (NADH) and focal marked pheophorbide acid 2 - deoxyglucose (Pyro-2DG) fluorescence intensity distribution. Methods: Using low-temperature imager (Cyro-imager) test of pancreatic islet cells of the redox properties observed within the pancreatic islet cells the accumulation of Pyro-2DG. Results: The pancreatic tissue obtained 2-D image maps, using three-dimensional visualization (Amira) Special software can be constructed medical 3-D high-resolution image. Conclusion: The findings help to assess the effectiveness of pancreatic tissue optics. [Keywords:] coke mark pheophorbide acid 2 - deoxyglucose, obese mice with diabetes, pancreatic cells, 3-D high resolution images Abstract Objective: To study fluorescence intensity distribution of Fp, NADH and Pyro-2DG in pancreatic islet cells and liver cells of obese-diabetic (ob / ob) mice. Methods: A cryo-imager was used to image the pancreas and liver and to examine the redox characteristics of the pancreatic islet cells and the accumulation of Pyro-2DG in pancreatic islet cells was observed. Results: High-resolution 3D images were constructed through 2D images of the serial sections of pancreas acquired using Amira medical software. Conclusion: This study helps to evaluate the validity of optical studies of pancreatic tissue. Key word Pyropheophorbide 2-dexyglucose (Pyro-2DG); Obese-diabetic mice; Pancreatic islet cells; High-resolution 3D images Diabetes is a metabolic disease, and less insulin secretion or insulin resistance caused by high blood sugar related. The cost of diabetes is very significant. The World Health Organization predicts that by 2025, the number of adults with diabetes worldwide will increase to 300 million , while the number of diabetic patients in China will reach