Of chlorogenic acid in chrysanthemum improved method for determination of.doc

 PAGE \* MERGEFORMAT 3 Of chlorogenic acid in chrysanthemum improved method for determination of [Keywords:] chrysanthemum;,, high-performance liquid chromatography;,, chlorogenic acid Abstract: The purpose of the Chrysanthemum in the determination of chlorogenic acid content in ways to improve it. Methods using high performance liquid chromatography (HPLC) determination of chlorogenic acid content. The results of chlorogenic acid in chrysanthemum content according to ‘The 2005 edition of Chinese Pharmacopoeia method Ⅰ Ministry of experiments, the complex sample extraction methods, the content will suffer large errors. Conclusions will be improved assay method with a sample solution extraction method is simple, the content is accurate and reliable, a good separation, high sensitivity, good stability and the advantages of the revised standard providing a viable basis. Keywords:: chrysanthemum; high-performance liquid chromatography; chlorogenic acid Chrysanthemum morifolium Ramat Chrysanthemum morifolium Ramat. Capitulum dry. Medicine according to the different origin and processing methods are divided into ‘bo’’Chu Ju Ju Gongju’’Chrysanthemums’’’. This daisy and chrysanthemum, respectively bo carried out content analysis, improved methods (2nd method) has a sample solution extraction method is simple, accurate and reliable content of excellent features. 1 Equipment Chloroform, phosphoric acid were analytically pure; methanol and acetonitrile were HPLC pure; Agilent 1100 HPLC ChemStation; Mettler AE240 electronic balance; samples from Hubei Province, Yichang City, Hubei Province Hong’an County beings pharmaceutical companies and the Chinese medicine hospital provided by the Yichang City Drug laboratory identified as ‘chrysanthemum’, provided by an enterprise in Hubei chrysanthemum was identified as ‘bo-ju’; chlorogenic acid reference substance (provided by the Central Inspection for the determination of use, batch number of 110,753,200,212). 2 Methods an