Of heparin on A549 cell growth and cell cycle status.docVIP

 PAGE \* MERGEFORMAT 10 Of heparin on A549 cell growth and cell cycle status Of: Chen Kefang Chenfang Lan Huang Longjie [Abstract] Objective To study the in vitro growth of dalteparin on pulmonary adenocarcinoma A549 cell activity and cell cycle. Methods 6 different concentrations (0,2,10,50,100 and 500 IU / ml) in the dalteparin interfere with the growth of A549 cells by MTT method at 4 time points (6,12,24 and 36 h) to detect cell activity; to normal after 24 h cultured in the presence of A549 cells to 50 IU / ml of heparin in the culture fluid in cultivation, cultivation of the cells as normal control group, two groups of cells after 24 h PI dye staining, and analyzed by flow cytometry cell cycle. Results dalteparin reduced A549 cell activity, and this effect showed Time dependent and concentration-dependent; of heparin inhibition of cells in G1 phase mainly. Conclusion dalteparin reduced on the A549 cell activity inhibition and cell cycle related. [Keywords:] The low molecular weight heparin; dalteparin; lung adenocarcinoma; Cell Cycle Heparin and its derivatives against cell proliferation, the current research on the muscle more. Guyton, etc. (1) reported that both in vivo or in vitro, heparin can reduce the smooth muscle cells (SMC) of mitosis. Other parts of the SMC, such as airway and intestine, myometrium and uterine fibroids can also be heparin inhibition of growth (2). but also on the endothelial cells on the antiproliferative effects of heparin, such as Khorana, etc. (3) reported that heparin Anti-microvascular endothelial cells (MVECs) and azygos vein endothelial cells. on the unrated heparin (UFH) or low molecular weight heparin (LMWH) on lung adenocarcinoma A549 cell growth inhibition, currently have less reported in the literature. The study choose one of heparin to LMWH research. 1 Materials and methods 1.1 Main Reagents and Instruments A549 cells (Experimental Center, Lanzhou University School of Medicine frozen). CO2 cell in