Of sodium selenite on rat mesangial cells expressed p38MAPK and MCP.docVIP

 PAGE \* MERGEFORMAT 8 Of sodium selenite on rat mesangial cells expressed p38MAPK and MCP [Keywords:] sodium selenite; p38 mitogen-activated protein kinase; monocyte chemoattractant protein Role of sodium selenite in regulating expressions of p38MAPK and MCP1 in rat mesangial cells [Abstract] AIM: To observe the effect of sodium selenite on expressions of p38 mitogenactivated protein kinase (p38MAPK) and monocyte chemoattractant protein1 (MCP1) in rat mesangial cell line HBZY1, and to study the mechanism of sodium selenite in preventing diabetic nephropathy. METHODS : Cell line HBZY1 was incubated with high glucose, high insulin, H2O2 and advanced glycosylation end products (AGEs), respectively (control group); simultaneously, the cell line HBZY1 pretreated with sodium selenite was also incubated with the above factors (experimental group) . The expressions of p38MAPK and MCP1 were detected and compared in the 2 groups. RESULTS: Four factors increased the expressions of p38MAPK and MCP1 indepently; sodium selenite inhibited the expressions of p38MAPK and MCP1 by the 4 factors distinctly. CONCLUSION: Sodium selenite can suppress the expressions of p38MAPK and MCP1 in the cell line HBZY1, which suggests that sodium selenite may play a significant role in the prevention of diabetic nephropathy by the inhibition of the expressions of p38MAPK and MCP1 in the cell line HBZY1. [Keywords] sodium selenite; p38 mitogenactivated protein kinase; monocyte chemoattractant protein1; mesangial cells; diabetic nephropathy [Abstract] Objective: To observe the sodium selenite on rat mesangial cell line HBZY1 expressed p38 mitogen-activated protein kinase (p38MAPK) and monocyte chemoattractant protein 1 (MCP1) influence, thus study of selenium in prevention and treatment of diabetic nephropathy (DN) in the mechanism of action. Methods: with high glucose, high insulin, hydrogen peroxide and advanced glycation end products (AGEs) stimulate the HBZY1 cell; the first to give 100