Of gossypol in cotton root bark extract and Process Optimization.docVIP

 PAGE \* MERGEFORMAT 11 Of gossypol in cotton root bark extract and Process Optimization 【Abstract】 Objective To establish the root bark of cotton, gossypol extraction technology and process were optimized. Ultrasonic extraction method using diethyl ether extraction of gossypol in cotton root bark, washing, concentration, gossypol obtained crude products; study of a variety of experimental conditions on the yield of gossypol, using spectrophotometric determination of the ingredients, as the evaluation of indicators. The optimum results for the ultrasonic extraction method. The cold soak 1 h, solid-liquid ratio (g * cm-3) 1:2.5, ultrasonic extraction 3 times, solid-liquid ratio 1:1,15 min / times, the temperature is below 15 ℃. Conclusions ultrasonic extraction process has the energy-saving, time-saving, high efficiency advantages. Keywords: cotton root bark; gossypol; Orthogonal design; Extraction Process Abstract: ObjectiveTo erect and optimize the extraction process of gossypol in cotton root bark. The gassypol was extracted from cotton root bark by the method of diethyl ether. Methods At the process of extraction, the effect of a variety of experimental conditions on yield of gossypol was studied and the content was determined by ultraviolet spectrophotometry.ResultsThe optimum extraction conditions were 1 hour soaking time, the ratio of material and solvent was 1:2.5, distilling 3 times with ultrasonic wave, controlling the temperature below 15 ℃. ConclusionThe uctrasonic extraction process the the advantages of less energy consumption, shorter time and high efficiency. Keywords:: Gossypol; Cotton root bark; Orthogonal design; Extraction process Cotton root bark, Malvaceae plants dry cotton root bark, there is qi, relieving cough and asthma features, for chronic bronchitis, physically weak edema, uterine prolapse and so on. Cotton root bark contains gossypol (gossypol) 1.5% ~ 2.5%, and contain asparagine and a small am