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Of taurine on rat limb ischemia-reperfusion injury after liver protective effect of
PAGE \* MERGEFORMAT 8
Of taurine on rat limb ischemia-reperfusion injury after liver protective effect of
Author: Wang Yinhuan, DONG Shu-yun, Zhang Na, Zhao Lijun, Hong-Jie Li, Xiao-Yan Kong, Li-Hua Liu, Lian-Yuan
[Keywords:] reperfusion injury; liver function; limbs; Taurine
Protective effect of taurine against liver injury after limb ischemiareperfusion in rats
[Abstract] AIM: To observe the liver injury and investigate the protective effect of taurine on the liver function after limb ischemiareperfusion. METHODS: Thirty Wistar rats were randomly divided into control group, ischemiareperfusion (IR) group and taurine ischemiareperfusion (TR) group ( n = 10 per group). The values of XOD, LDH, MDA, AST, ALT and SOD in plasma were respectively measured after 4 h limb ischemia and 4 h reperfusion in each group. XOD, MDA, ROS, MPO and [Ca2] in the liver tissues and GSHPX, [Ca2] in the liver mitochondria were observed. RESULTS: In IR group compared with the control group, the values of LDH [(190.16 ± 13.36) μ kat / L vs (122.39 ± 14.87) μ kat / L ], XOD [(758.82 ± 151.53) nkat / L vs (543.61 ± 43.51) nkat / L], MDA [(5.19 ± 0.67) nmol / L vs (1.27 ± 0.21) nmol / L], ALT [(78.40 ± 6.45 ) nkat / L vs (20.50 ± 3.70) nkat / L], AST [(47.70 ± 4.47) nkat / L vs (25.25 ± 2.98) nkat / L] in plasma increased, but SOD [(1.30 ± 0.15) μ kat / L vs (1.80 ± 0.16) μ kat / L] reduced, XOD [(104.69 ± 12.34) μ kat / kg vs (59.01 ± 10.50) μ kat / kg], MDA [(2.66 ± 0.08) nmol / L vs (1.29 ± 0.14) nmol / L], ROS [(771.65 ± 100.69) μ kat / L vs (606.45 ± 52.01) μ kat / L], MPO (0.47 ± 0.04 vs 0.28 ± 0.06), [Ca2] [(0.248 ± 0.050) mmol / L vs ( 0.123 ± 0.014) mmol / L] in the liver tissues increased, GSHPx [(20.34 ± 4.67) nkat / L vs (31.17 ± 11.50) nkat / L] in the liver mitochondria were obviously reduced, but [Ca2] [(0.38 ± 0.06) mmol / L vs (0.14 ± 0.03) mmol / L] increased; In TR group compared with the IR group, the values of LDH [(158.29 ± 4.87) μ kat
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