Oil Preservatives in food by RP-HPLC determination of.doc

 PAGE \* MERGEFORMAT 7 Oil Preservatives in food by RP-HPLC determination of [Keywords:] preservative testing For the Oil preservative in food testing, GB GB/T500929-2003 not expressly provided for processing and testing methods, laboratory generally use the acidic ether extraction, enrichment, gas chromatography. This method is due to the adsorption and column packed column performance vary greatly, so that the standard series linear deviation, the minimum detection limit of high dilution for the content of low or large samples can not be detected. This method uses sodium hydroxide mixture of ethanol extraction, reversed-phase liquid chromatography UV detector and the effective elimination of emulsion extraction phenomenon, to improve analytical sensitivity and accuracy. An instrument and reagent 11 main instruments L7000-based high-performance liquid chromatography system (Hitachi HITACHI Corporation), with L7420 model UVVis detector, D7000 chromatographic data processing system; 802 centrifuge (Shanghai Surgical Instruments Factory); XK96B Fast-Mixer (Jiangyan New Kang Medical Device Co., Ltd.). 12 reagent benzoic acid and sorbic acid standard stock solution: 100mg/ml (National Research Center for Certified Reference Materials); 001mol / L sodium hydroxide ethanol solution; 008mol / L hydrogen chloride solution; 002mol / L ammonium acetate solution; petroleum ether and ethyl ether mixture: petroleum ether (AR) ether (AR) = 3 1; methanol: HPLC pure. 13 Instrument condition column: Kromasil C18 (250 * 46mm, ID5μ m); mobile phase: methanol 002mol / L ammonium acetate solution = 595; flow rate: 10ml/mim; injection volume: 5μ l; column temperature: room temperature; detection wavelength: 230nm. 14 Methods (1) Sample processing: an accurate, said the samples taken after mixing 100g of a plug in 10ml centrifuge tube, add 50ml of petroleum ether and ether mixture, with rapid mixer to dissolve grease. Join 001mol / L sodium hydroxide ethanol solution of 40ml,