Of paeoniflorin in Radix Paeoniae Rubra choice of extraction solvent.docVIP

 PAGE \* MERGEFORMAT 8 Of paeoniflorin in Radix Paeoniae Rubra choice of extraction solvent [Keywords:] TPG;,, paeoniflorin;,, high-performance liquid chromatography Abstract: Objective To study the drug glycosides extracted from Paeonia best solvent. Methods were used methanol, 75% ethanol, re-distilled water extract of paeoniflorin red medicine, with YWG-C18 (250 * 4.6 mm) column with methanol -0.05 mol / L potassium dihydrogen phosphate solution (32:68) as mobile Determination of paeoniflorin phase, detection wavelength was 230 nm. Results In the test concentration (0.010 ~ 0.200 μ g / μ l) a good linear relationship range of r = 0.999 8, n = 5; with methanol, 75% ethanol, re-distilled water as solvent Paeonia glycoside contents were 3.01%, 3.09% , 3.07%. Conclusion The results showed that methanol, 75% ethanol, re-distilled water as solvent extraction and concentration of paeoniflorin in Paeonia no significant difference (Pamp;gt; 0.05); the use of distilled water as solvent to avoid the toxicity of methanol, strong and inexpensive; we believe that the use of re-distilled water as solvent is superior to ethanol, methanol. Keywords:: Paeonia; paeoniflorin; high-performance liquid chromatography Effects of Different Solvents on the Extraction of Paeoniflorin from Radix Paeoniae Rubra AbstractObjectiveTo select the optimal solvent to extract paeoniflorin from Radix Paeoniae Rubra.MethodsPaeoniflorin was extracted from Radix paeoniae Rubra with methanol, 75% ethanol and redistilled water, respectively. Paeoniflorin was separated on a YWG-C18 (250 mm * 4.6 mm) using methanol: 0.05 mol / L KH2PO4 (32:68) as a mobile phase and determined at 230 nm.ResultsThe linear curve was ideal within the tested range of 0.010 ~ 0.200 μ g / μ l (r = 0.9998, n = 5); the average content of paeoniflorin extracted with methanol, 75% ethanol and redistilled water was 3.01%, 3.09%, 3.07%, respectively.ConclusionThere was no significant difference in extraction of paeoniflorin wi