Olfactory ensheathing cells activated the role of astrocytes.doc

 PAGE \* MERGEFORMAT 18 Olfactory ensheathing cells activated the role of astrocytes Of: Liu Yuan, Liu Jinbo, Zhang Zhijian, Gong Aihua, Zhang Jun [Abstract] Objective: To study the in vitro cultured rat olfactory ensheathing cells (olfactory ensheathing cell, OEC) on the activation of astrocytes (astrocytes, AST) role. METHODS: Cultured olfactory ensheathing cells and astrocytes . astrocytes purified passage, the cell fusion method using scratch astrocytes are activated, the activation of olfactory ensheathing cells and astrocytes were cultured 24 h, astrocytes observed changes in proliferation and the expression of glial fibrillary acidic protein (GFAP) and chondroitin sulfate proteoglycan (CSPG). Results: Activation of astrocytes and olfactory ensheathing cells were cultured, MTT test showed that the proliferation of capacity enhancement; immunofluorescence staining, RT-PCR and Western blot test showed that the activation of co-cultured astrocytes decreased the expression of GFAP and CSPG. Conclusion: Olfactory ensheathing cells in activated astrocytes after , can promote the proliferation of astrocytes, while able to reduce astrocyte gliosis, decreased expression of inhibitory cytokines. [Keywords:] olfactory ensheathing cells; astrocytes; proliferation; glial fibrillary acidic protein (GFAP); chondroitin sulfate proteoglycan (CSPG) [Abstract] Objective: To study the effect of olfactory ensheathing cells on reactive astrocytes in vitro.Methods: Olfactory ensheathing cells and astrocytes were cultured from Sprague Dawley Rats in vitro. Astrocytes were purified then passaged and grown to confluence.Some cultures were scratched to induce a reactive astrocyte phenotype.Olfactory ensheathing cells were added onto confluent astrocytes for 24 h.The differences of cell proliferation, expressions of glial fibillary acidic protein (GFAP) and chondroitin sulfate proteoglycans (CSPG) were measured among the normal group, active group, and the co cultured gr