Oxidized glutathione on free radical scavenging glutathione synergy.doc

 PAGE \* MERGEFORMAT 14 Oxidized glutathione on free radical scavenging glutathione synergy Of: Jin Chunying Cuijing Lan Cui Shengyun [Abstract] using spectrophotometry and matrix-assisted flight mass spectrometry studies on 1,1-diphenyl-2 glutathione bitter hydrazine (DPPH) radical scavenging effect. By comparing the different concentrations and different proportions of reduced glutathione (GSH) and oxidized glutathione (GSSG) and mixed solution Na2SeO3 radical scavenging, found GSH / GSSG ratio of free radical scavenging rate had a significant effect. When the GSH / GSSG ratio of greater than 50:1, the radical scavenging large compared with the same concentration of GSH, and the radical scavenging with the absolute concentration of GSH and GSSG was significantly increased with the increase, indicating that the right amount of GSSG GSH can eliminate free radicals during cooperative catalysis. MS test showed that: the synergy and GSSG in the process of free radical scavenging reactions relevant. Na2SeO3 GSH scavenging of free radicals on the main reaction GSSG with GSH to regulate the GSH / GSSG ratio results. by measuring and analysis of a certain ratio of GSH + GSSG before and after mixture with the mass spectra of DPPH was proposed under the coexistence of a small amount of GSSG, GSH DPPH free radical catalytic mechanism of removal. [Keywords:] glutathione, free radicals, antioxidant activity, spectrophotometry, matrix-assisted flight mass spectrometry 1 Introduction Glutathione is the antioxidant defense system in vivo the most important activity of small molecule oligopeptide, into oxidized glutathione (GSSG) and glutathione (GSH) [1]. GSH as the glutathione glutathione peroxidase (GPx) or glutathione S-transferase (GSH s transferase) of the substrate, can remove harmful free radicals in vivo (mainly free radicals) or lipid peroxides, to convert into fatty acids and water, and GSH is oxidized to GSSG [2] (see diagram 1). diagram 1 GSH antiox