Oyster liquid antioxidant enzyme activity of.doc

 PAGE \* MERGEFORMAT 16 Oyster liquid antioxidant enzyme activity of Author: Dan-Qing Zhou Xian-guo Lei Zhang Hui Yang Qin [Abstract] Objective To investigate the oyster liquid antioxidant enzyme activity. Methods Determination of Prussian blue reaction method to restore the oyster hydrolyzate capacity in DPPH system, Fenton system and pyrogallol auto-oxidation system, were used to detect clear diphenyl-generation oyster hydrolyzate bitter acyl radical (DPPH * ), hydroxyl free base (* OH) and superoxide anion (O2-* ) capacity, and examine oysters hydrolyzate than in the Fe2-induced egg-yolk lipoprotein polyunsaturated fatty acids (PUFA) peroxidation system, the anti-oxidation. Results oyster hydrolyzate clear DPPH * and OH, EC50, respectively 7.313 and 1.330 mg / ml; oyster hydrolyzate (17.800 mg / ml) on superoxide radical and yolk lipoprotein lipid peroxidation inhibition rates were 19.86% and 82.97%. Conclusion oyster hydrolyzate has a significant role in removal DPPH * and OH, and the activity was positively correlated with the dose, and egg yolk lipoprotein lipid peroxidation also showed strong inhibition. [Keywords:] oyster enzymatic Antioxidant Modern medical studies have shown that an appropriate amount of free radicals in the immune response, cell differentiation, apoptosis and other biochemical metabolism plays an important role, but an excessive amount of free radicals in the body caused a series of biological reactions, leading to tissue cells , sub-cellular and molecular structure of the damage, and with the level of damage caused by the gradual expansion of functional damage caused by cardiovascular diseases, cancer and aging, etc., which constitute the pathological basis of many diseases. Therefore, rational use of anti-oxidants can be effective in the prevention and treatment of certain diseases, anti-aging. Synthetic anti-oxidants tend to have certain toxic side effects, making their use is strictly controlled. The natural anti-oxidants