P21 mRNA expression in prostate cancer and lymph node metastasis.docVIP

 PAGE \* MERGEFORMAT 7 P21 mRNA expression in prostate cancer and lymph node metastasis [Abstract] Objective To study the primary expression of p21 in prostate cancer, and explore its clinical significance in prostate cancer. Methods RT PCR method to detect prostate cancer and p21 expression in adjacent tissues. Results in 67 patients with prostate cancer , 31 cases (46.29%) expressed p21 mRNA; adjacent normal tissue, 49 cases (73.13%) expressed p21mRNA. to prostate cancer were divided into 2 groups, respectively, with lymph node metastasis and no lymph node metastasis. In 41 cases with regional lymph node metastasis in, p21 mRNA expression in 13 cases (31.71%). In 26 cases without lymph node metastasis cases, p21 mRNA expression in 18 cases (69.23%). Conclusion p21 in primary prostate cancer tissues and adjacent noncancerous significantly reduced compared with normal tissue; P21 expression in lymph node metastases was significantly lower than those without lymph node metastasis. [Keywords:] p21; prostate cancer; tumor suppressor gene p21 is the most widely known to have CDK inhibitory activity of cell cycle control protein, negative regulator of CDK function indicates its role in tumor suppression. P21 and cancer development and biological behavior, may be estimated malignant tumor behavior and prognosis of new indicators. The application of molecular biology experiments designed to detect and analyze the primary expression of p21 in prostate cancer, and explore its clinical significance in prostate cancer. 1 Materials and methods 1.1 Organization and reagents collected from January 2007 to July 2008 67 cases of hospital urology cancer surgery prostate cancer tissues and adjacent normal tissue. Sent immediately after surgery -80 refrigerator frozen deep hypothermia. All cases of distant metastasis were not found before surgery, no radiotherapy and chemotherapy, patients were pathologically confirmed, primary prostate cancer are. Trizol was purc