Particle toxicology studies Dingchuan.doc

 PAGE \* MERGEFORMAT 8 Particle toxicology studies Dingchuan Authors: Xiao-hua, Zhu Ling-yuan, Xu Liying, Ding Qi, Guo Sheng, Fang Aluminum [Abstract] Objective To observe the mice and rats were given gavage Dingchuan acute toxicity of particles and long-term toxicity. Methods Bliss Determination of LD50 in mice fed Dingchuan particles, as well as in rats fed a continuous Dingchuan particles 60 d, observe the administration of 60 d of growth, development, hematology, blood biochemistry, histopathological changes and withdrawal 21 d, the above-mentioned indicators. Results of Kunming mice fed Dingchuan particles LD50 of 171.09 g / kg. Dingchuan particles in rats administered 60 d toxicity tests, dose, respectively 72.6,36.3,18.2 g / kg (refer to the amount of crude drug containing the same below) (equivalent to the clinical dosage of 50 kg were 52.6,26.3 and 13.2-fold), rats in each group during the administration of fur, activities, two have no anomalies, but the administration of rats feeding, drinking, weight and some hematology, blood biochemical indicators and the organ coefficient of abnormal changes, after stopping, The abnormal change indicators have returned to normal. Delivery period and the recovery of important organs pathological examination were not found significant anomalies. Conclusion Dingchuan particles in mice fed the LD50 of 171.09 g / kg; long-term toxicity studies have shown that long-term high-dose gavage in rats given Dingchuan particles have a certain toxicity, but have resumed after treatment. [Keywords:] Dingchuan particle long-term toxicity test Acute toxicity test Abstract: Objective To observe the acute toxicity of Dingchuan granule (DCG) in mice and the long-period toxicity in rats, respectively.MethodsLD50 of DCG were tested in mice by Bliss methods.Rats were administered with repeated gastric infusion of DCG for 60 days and body weight, hematological and hematobiochemical parameters, coefficient and histomorphological figure