PCR detection of minimal residual leukemia in children Study.doc

 PAGE \* MERGEFORMAT 16 PCR detection of minimal residual leukemia in children Study Abstract minimal residual leukemia (MRD) refers to the leukemia after complete remission after chemotherapy, there are still forms in the bone marrow can not be detected on leukemia cells, is the main reason for relapse. Children’s MRD detection in judging the prognosis of childhood leukemia, the development of leukemia treatment programs are important. MRD detection PCR method is rapid, specific, simple, economic, and samples of less and so on. This paper reviews the recent years, PCR screening of multiple target genes of leukemia in order to fluorescence quantitative PCR (FQ  PCR) to track the progress made with regard to MRD. Keywords: PCR minimal residual leukemia, multiple PCR FQ  PCR childhood leukemia Detection of Minimal Residual Disease in Children Leukemia Patients by Using PCR - Review Abstract MRD detection in children leukemia has a potential importance to predict clinical outcome and to modify treatment protocols of the diseases. Although some patients with leukemia have achieved complete remission according to the clinical and morphological criteria, there are still very low numbers of malignant cells that can not be discriminated by morphology and remained in bone marrow, which is called minimal residual disease (MRD) and is the main reason leading to relapse. MRD detection has an important significance for designing treatment protocols. Several methods of MRD detection have been developed. These include conventional cytogenetics, fluorescence in situ hybridization (FISH), flow  cytometric immunophenotyping (FCM), Southern blot and polymerase chain reaction (PCR) techniques, etc. Each of these techniques has its advantages and disadvantages, so not all of them are suitable for clinical MRD detection because of several inherent disadvantages, such as limited sensitivity, time  consuming, high cost, or requiring high  quality DNA o