PCNA of the shRNA eukaryotic expression vector and pairs of cervical cancer intervention study.doc

 PAGE \* MERGEFORMAT 6 PCNA of the shRNA eukaryotic expression vector and pairs of cervical cancer intervention study Author: Huang Hao Xin Tu, Nan-Cai Yu, Wen-Li Wu, Qian Liu, Ma Wei, Hao Jianjun, Yi Yan-dong [Abstract] Objective To construct PCNA small hairpin RNA (shRNA) eukaryotic expression vector and in Hela cells, while observing the PCNA of shRNA on the proliferation of human Hela cell lines in vitro and biological characteristics of the impact of change. Methods PCNA of the cDNA of the shRNA primers were inserted into eukaryotic expression vector pGenesil  1, eukaryotic expression plasmid pGenesil  1  PCNA1  4, and through digestion and sequencing methods were identified, the eukaryotic expression plasmid pGenesil  1  PCNA1  4 transfected cervical cancer cells, using Western blot detection of PCNA protein expression, flow cytometry analysis of cell cycle. The results of PCNA in shRNA can significantly inhibit the growth of Hela cells, blocking cell G0/G1-S phase, PCNA protein expression was inhibited at the same time. Conclusion PCNA of shRNA can significantly inhibit the growth of Hela cells, its inhibition may be achieved by blocking the expression of PCNA protein in the experimental results of the shRNA for further study PCNA gene therapy of cervical cancer laid the foundation. [Keywords:] cervical cancer, proliferating cell nuclear antigen, a small hairpin RNA cervical cancer Constructed Eukaryotic Expression Plasmid of Small Hairpin RNA (shRNA) of PCNA and Investigated the Inhibitory Effect on Hela Cell Keywords:: Uterine cervix carcinoma; Proliferating cell nuclear antigen; Small hairpin RNA; Hela cell The occurrence of cancer and genetic mutations and cell proliferation. Proliferating cell nuclear antigen (Proliferating cell nuclear antigen, PCNA) is a widely expressed cell cycle S phase of an attack of gout, is a proliferation of cells only in the synthesis and expression of 36KD polypeptide and its function is an important