PEI alone and with three kinds of antimicrobial agents in conjunction with the in vitro antibacterial activity of Candida albicans.doc

 PAGE \* MERGEFORMAT 15 PEI alone and with three kinds of antimicrobial agents in conjunction with the in vitro antibacterial activity of Candida albicans Author: Chen Tao LU Ting-li ZHANG Yan Fu Ching-kuo [Abstract] Objective To evaluate the multi-branched-chain cationic polymers - polyethylene imine (PEI) alone and with three different types of conventional antimicrobial agents (amphotericin B, fluconazole, and polymyxin B) joint use of Candida albicans (MY7245 and MY7238) clinical isolates of the two kinds of anti-fungal activity. Methods The in vitro determination of different molecular weight polyethylene imine alone and with three different types of conventional antimicrobial agents combined use of two Candida albicans minimal inhibitory concentration and kill the time, to determine the separate and joint use of PEI in vitro anti - antifungal activity. 2 ~ 745ku results of the molecular weight PEI within the scope of these two kinds of Candida albicans are a very good anti-fungal activity, and the molecular weight of PEI smaller than the molecular weight of PEI antibacterial effect more pronounced. Compared with the single action of PEI, the PEI molecular weight greater when used with conventional drugs in combination can produce a strong synergistic effect. Lethal studies in vitro experiments can be seen, PEI-amphotericin B, fluconazole-PEI-conjugates can increase the antimicrobial effect, but the PEI-conjugates of polymyxin B is the role of antagonistic bacteria. Conclusions PEI alone with joint use of both Candida albicans produce good anti-bacterial effect. [Keywords:] polyethylene imine; antibacterial drugs; Candida albicans; in vitro antifungal activity of ABSTRACT Objective To evaluate the antifungal activity of branched synthetic polymer, polyethylenimine (PEI), alone and in combination with different antimicrobial agents against two clinical isolates of Candida albicans (MY7458 and MY7238). MethodsThe minimal inhibitory concentration (MIC) an