People Lrp polyclonal antibody.doc

 PAGE \* MERGEFORMAT 10 People Lrp polyclonal antibody Author: Yu Xin-Ping, Song to celebrate, HOU Li-chao, DU Ke-jun, XIONG Li-ze, CHEN Su-min, CHEN Nan-chun, on behalf of Tadaaki [Abstract] Objective: To prepare human Lrp polyclonal antibodies. Methods: The cloning of full-length lrp  cDNA coding sequence and make Prokaryotic expression and identification. Using Ni-chelating column (Ni  NTA) purification of prokaryotic expression of the full-length Lrp protein, to obtain high purity of the target protein. with the purified protein immunization of New Zealand white rabbits, and polyclonal antibody preparation adsorbed to respond unless the specific ingredients. Results: Western blot results showed that the purified anti-serum before and after the 69 ku were detected at the target protein band, indicating adsorption of the purified antibodies can specifically bind with the Lrp protein. while the purified antibody Western blot bands purpose is only one, indicating the high adsorption obtained after purification of specific anti-serum, Western blot of the effect of its price in the 10-6 above, there is a higher titer Western blot. CONCLUSION: The highest bid was prepared by high-performance specific rabbit anti-human polyclonal antibodies Lrp protein for further study of Lrp function provides an important experimental tool. [Keywords:] Lipopolysaccharides 0 Introduction LPS (lipopolysaccharide, LPS) is a bacterial endotoxin, is a Gram-negative (G-) bacterial infections, the main pathogenic factor, it is closely related to the occurrence of certain inflammation [1]. Explore the altered expression after LPS stimulation of gene function, targeted study in LPS signal transduction pathway in the role of the location and role in nature, will help to increase understanding of molecular mechanisms of inflammation. who lrp (lipopolysaccharide responsed gene, lrp) is a kind of fat polysaccharide response gene. Department of the research group improved PCR-based su