Phage random 12-mer peptide library VEGF mimotope screening and preliminary identification of.docVIP

 PAGE \* MERGEFORMAT 14 Phage random 12-mer peptide library VEGF mimotope screening and preliminary identification of [Abstract] Objective: From the random 12-peptide phage library was screened with the anti-vascular endothelial growth factor (VEGF) mAb Avastin (Avastin) specific binding peptides. Methods: Avastin for the target molecules on the phage peptide library 12 3 bio-panning, randomly picked 80 clones, ELISA were identified by their affinity for the high-affinity clones, DNA sequence analysis, deduced with the shell integration of phage peptide 12 amino acid sequence. Fmoc solid-phase synthesis of 12 peptide, glutaraldehyde cross-linked 12-peptide with hemocyanin (KLH), dot blotting (Dot blot) detection conjugates can combine with Avastin. Results: ELISA showed that 42 phage clones with Avastin have stronger pro - and the nature of the sequence was found 41 positive clones expressed fusion peptide sequence in line 12, a different; chemically synthesized 12 peptides with the Avastin-specific binding, 12 peptides  KLH conjugates can specifically bind with Avastin. Conclusion : Preliminary evidence part of the 12-peptide analogue of the VEGF epitope. [Keywords:] Avastin (bevacizumab) bacterial phage peptide library of vascular endothelial growth factor cross-linked 0 Introduction Angiogenesis is a prerequisite for tumor growth and metastasis [1]. Vascular endothelial growth factor (VEGF) is to promote blood vessel formation in the key factors [2-3]. Expression level of VEGF in tumor tissue and tumor blood vessels of the degree of the degree of malignancy a positive correlation [4-5]. Thus, VEGF is a tumor anti-angiogenesis therapy in the ideal target molecule. phage random peptide library containing a large number of structural polypeptides of different sequences can be used as any of the target epitope mimic peptide, the use of peptide library screening the target protein does not need any structure, just the target antigen mAb can get is immunogen