Phosgene inhalation on rat lung tissue gene expression profiles of.docVIP

 PAGE \* MERGEFORMAT 12 Phosgene inhalation on rat lung tissue gene expression profiles of [Abstract] Objective: To study the phosgene inhalation in rat lung tissue before and after the differences in gene expression to explore the role of phosgene lung injury-related genes spectrum. Methods: Extraction of the normal control group and phosgene lung tissue total RNA, and Preparation of fluorescently labeled this as a template for cRNA target material, after hybridization, washing, scanning through the fluorescence intensity and computer software analysis, looking upward and downward exposure before and after the differentially expressed genes. Results: 20500 target genes in the rat, the initial screening out of 801 differentially expressed genes, there were 557 upregulated and down there are 254. According to the biological function of genes, differentially expressed genes were divided into nine categories: G1 radical \ electron transport-related; G2 inflammatory stress -related; G3 metabolism-related; G4 cell proliferation \ Differentiation \ apoptosis-related; G5 regulation of gene expression-related; G6 receptors and signal transduction related; G7 protein modification \ decomposition-related; G8 cytoskeleton \ sports-related; G9 ion channels and such as transport-related substances. Conclusion: phosgene can cause changes in lung tissue of multiple genes, their functional classification of differentially expressed genes phosgene poisoning in order to further explore the occurrence and development of mechanisms and effective treatment to provide new clues and ideas. [Keywords:] phosgene; lung / injury; Oligonucleotide Array Sequence Analysis; gene expression Phosgene (phosgene, COCl2) also known as carbonyl chloride, are highly toxic suffocating agents, synthetic chemical products, an important raw material, widely used in paints, pesticides, plastics and textile production, due to volatile low temperature (8.2 ℃ ) , high dispersion, the reaction quickly, b