Picrorhiza cerebral ischemia in rats the expression of nerve growth factor.doc

 PAGE \* MERGEFORMAT 10 Picrorhiza cerebral ischemia in rats the expression of nerve growth factor [Abstract] Objective To observe the ischemia-reperfusion (I / R) rat brain nerve growth factor (NGF), and to explore Picrorhiza protective effect on neurons. Methods patching method in the light of cerebral I / R model, Picrorhiza intervention was observed by immunohistochemistry cortex, striatum NGF expression. ischemic model group cortex and striatum in the area in I / R 3 d to a peak when the NGF expression, 7 d after the gradual decline But each time point compared with the sham group, there were significant differences (P lt;0.01); Picrorhiza treatment of rat brain cortex and striatum of NGF was significantly higher than the normal group and model group (P lt;0.01). Conclusion Picrorhiza cerebral I / R injury in neurons has a protective effect, which may increase the brain with NGF expression. [Keywords:] Picrorhiza; medicine; cerebral ischemia; nerve growth factor; rat [Abstract] Objective To observe the expression of nerve growth factor (NGF) in ischemic reperfusion (I / R) rat brain and investigate its mechanism. Methods The cerebral I / R models in rats were made by intraluminal thread method and interfered by picrorhizae. The expression of NGF in cortex and striatum were determined by immunohistochemistry. Results In model group, the expression of NGF peaked at 3 day on ischemic cortex and striatum and descended gradually after 7 days. But compared to model group, there was significant difference at each time point (P lt;0.01). In rat cortex and striatum of picrorhizae treated group, the expression of NGF positive neurons was higher than that of sham operated group and model group significantly (P lt;0.01). Conclusions Picrorhizae can protect the neuron from I / R injury by increasing the expression of NGF in brain. [Keywords:] Picrorhizae; Traditional Chinese medicine; Cerebral ischemia; Nerve growth factor; Rats Nerve growth factor (NGF)