Platelet-derived growth factor and insulin-like growth factor on the impact of lumbar disc degeneration.doc

 PAGE \* MERGEFORMAT 14 Platelet-derived growth factor and insulin-like growth factor on the impact of lumbar disc degeneration [Abstract] Objective: To investigate the platelet-derived growth factor (PDGFs and insulin-like growth factors (IGFs on rat intervertebral disc degeneration (IDD. Methods: 36 healthy adult SD rats were divided into PDGFs experimental group, PDGFs Canada IGFs experimental group and negative control group 3 group, IDD model established by surgical methods. In different times after injection of the 3 groups were PDGFs, PDGFs added IGFs and physiological saline, after modeling a month and 2 months in each group were 6 rats were sacrificed and complete remove the L3 ~ 4 disc measuring proteoglycan content, take L4 ~ 5 intervertebral disc using the immunohistochemical assay of type collagen content, take L5 ~ 6 disc were detected by flow cytometry using rat intervertebral disc cells apoptosis. Results: The modeling and 1 month after the date of the beginning of the 3 groups injected reagent disc proteoglycan content, collagen content and cell apoptosis rate differences were statistically significant (P lt;0.05. Conclusion: PDGFs rats with prevention and treatment of IDD, PDGFs coupled with the synergistic effect of IGFs. [Keywords:] disc, degenerative changes, platelet derived growth factor, insulin-like growth factor, rat Disc degeneration (intervertebral disc degeneration, IDD) is lower back pain and lumbar disc herniation mainly due to [1], clinical conservative treatment and surgical treatment are not ideal [2]. With the biochemistry, molecular biology the development of the relationship between growth factor and IDD much the concern of scholars home and abroad [3]. platelet-derived growth factor (PDGFs content of the human body as a little and a variety of biologically active peptides, the source of mesenchymal The cells have a strong promote cell division, promoting the role of matrix synthesis, is one of important cytokines