PMP12 polysaccharide Purification and Structure Analysis.docVIP

 PAGE \* MERGEFORMAT 15 PMP12 polysaccharide Purification and Structure Analysis Of: Zhao, Chang Yu, Pei-Hong, Zhang Lei, OUYANG Zhen [Abstract] Objective: To obtain purified from mulberry leaves homogeneous polysaccharides PMP12, and to study their composition and initial structure. Methods: mulberry leaves by hot water extraction ethanol precipitation, removal of protein, bleaching, by DEAE cellulose and Sephadex G 100 gel column chromatography to obtain a homogeneous polysaccharide PMP12, using gas (GC), high performance liquid (HPLC), infrared (IR), nuclear magnetic resonance (N