PMP12 polysaccharide Purification and Structure Analysis.doc

 PAGE \* MERGEFORMAT 15 PMP12 polysaccharide Purification and Structure Analysis Of: Zhao, Chang Yu, Pei-Hong, Zhang Lei, OUYANG Zhen [Abstract] Objective: To obtain purified from mulberry leaves homogeneous polysaccharides PMP12, and to study their composition and initial structure. Methods: mulberry leaves by hot water extraction ethanol precipitation, removal of protein, bleaching, by DEAE cellulose and Sephadex G 100 gel column chromatography to obtain a homogeneous polysaccharide PMP12, using gas (GC), high performance liquid (HPLC), infrared (IR), nuclear magnetic resonance (NMR), Smith degradation and reduction of uronic acid and other methods to analyze the composition and the initial structure. Results: PMP12 from the rhamnose (Rha), arabinose (Ara), galactose (Gal) and glucuronic acid (GluA), whose molar ratio of Rha: Ara: Gal: GluA = 1:1.56: 1.57:1.08; PMP12 main chain mainly 1 3 glycosidic linkage of the rhamnose side chains mainly 1 2 glycosidic bond and the 1 4 glycosidic linkage of arabinose, galactose and glucuronic acid. Conclusion: The determination of the polysaccharide composition and initial structure of PMP12, as the polysaccharide to provide the basis for further research. [Keywords:] mulberry leaves; polysaccharide; purification; Structure [Abstract] Objective: To study the isolation, the composition and characterization of polysaccharide PMP12 from Mulberry leaves. Methods: Mulberry leaves were extracted by boiling water. The polysaccharide in the filtrate was precipitated fractionally by alcohol. The protein in the product was removed by TCA and it was further purified by DEAE ion exchange cellulose (DEAE 52) and SephadexG 100, received PMP12.The composition and characterization of Mulberry leaves polysaccharide were researched by GC, HPLC, IR, 1H NMR, Smith degradation and uronic acid reduction and so on. Results: PMP12 is made up of rhammose, arabinose, galactose and glycuronic acid with the molarity rate of 1:1.56: