Piroxicam increased -T cell killing mechanism of gastric cancer cells.doc

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2017-05-05 发布于浙江
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Piroxicam increased -T cell killing mechanism of gastric cancer cells.doc

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Piroxicam increased -T cell killing mechanism of gastric cancer cells

Piroxicam increased ?T cell killing mechanism of gastric cancer cells Of: Ting Wang, tenda, Chen revival, Tao Zheng, the Lvxiao Ting, Li Yi, Zhang Song, Liu Jun rights [Abstract] Objective �æ�T Piroxicam increased cell killing mechanism of gastric cancer cells. Methods �æ�T cells cultured by conventional methods; 9 days in culture �æ�T cells with different concentrations of piroxicam (respectively 0.01,0.02,0.04,0.08, 0.16 mmol / L) induced, 24 h after the supernatant collected for cytokine interferon- (IFN-), tumor necrosis factor-@ (TNF-@), interleukin 12 (IL-12) test, precipitation cells measured by flow cytometry perforin, granzyme B and LDH detected by NKG2D and �æ�T cell killing activity of gastric cancer cells. Results Piroxicam concentration was 0.04 mmol / L when the �æ�T cells induced by perforin and granzyme B were 78.7 % and 71.8%, significantly higher (respectively 51.4% and 60.9%). Piroxicam significantly inhibited �æ�T cell NKG2D expression and with the drug concentration effect of the more obvious. �æ�T cells were treated with Piroxicam induced 24 h After the supernatant IFN- and IL-12 concentrations compared with the control group did not change; but can inhibit the secretion of TNF-@, and with the increase of drug concentration inhibited more obvious. �æ�T BCG cell killing cancer cells -823 activity in the 0.04 mmol / L when the highest (75%), significantly higher (58%). Conclusion Piroxicam �æ�T induced cell killing activity of BCG-823 significantly increased the expression may be related to higher �æ�T concentrations of perforin and granzyme B related. [Keywords:] �æ�T cells; piroxicam; Perforin; granzyme B; NKG2D; -interferon; @ - tumor necrosis factor; Interleukin 12; gastric cancer Abstract: Objective To explore the effect of piroxicam in enhancing the cytotoxicity of human �æ�T cells to gastric cancer cells. Methods �æ�T cells were routinely cultured and collected on the 9th day, followed by induction with piroxicam at different c