Pollution and more heparin sulfated chondroitin sulfate in the sub-step analysis of cellulose acetate electrophoresis.docVIP

 PAGE \* MERGEFORMAT 16 Pollution and more heparin sulfated chondroitin sulfate in the sub-step analysis of cellulose acetate electrophoresis Study: Gap Wang Hao in the Kwong Lee Hao Zhao Yan Xiuhong Cui Hu Yannan [Abstract] ion exchange chromatography to separate from the contaminated raw heparin sulfated chondroitin sulfate from multiple (OSCS), established step by step analysis of cellulose acetate electrophoresis content OSCS contaminated heparin. Results show that, first to 0.05 mol / L barium acetate buffer (pH 5.0) electrophoresis, and then to 0.15 mol / L zinc acetate buffer (pH 6.3) electrophoresis, can be completely separate heparin and OSCS detection limit of 0.1 g / L; through the gray points establish a quantitative calibration curve correlation coefficient of 0.9934, the average recovery was 102.1% ~ 106.1%; RSD was 4.1% and 6.0%. [Keywords:] Heparin, chondroitin sulfate, cellulose acetate electrophoresis 1 Introduction Heparin (heparin, HP) as an anticoagulant widely used in clinical [1,2], the common adverse reactions .2008 only the beginning of bleeding or thrombocytopenia, the clinical application of HP United States injection more serious allergic reaction occurred, presumed to be the raw material contains more than HP sulfated chondroitin sulfate (oversulfated chondroitin sulfate, OSCS) on [3], but the lack of sufficient evidence. To enhance the quality of such raw materials, supervision, and establish a rapid and effective detection of HP in OSCS approach is necessary. At present, the detection of HP’s common methods are biological methods [4], spectrophotometry [5,6], light scattering [7], electrochemical analysis [8], HPLC method [9] and capillary electrophoresis [10]. but OSCS and the HP structure is very similar to conventional biological methods can not detect them, and capillary electrophoresis (CE) and nuclear magnetic resonance (NMR) and other methods [3,11] Although you can use in OSCS detection, but there is equipme