Potentilla discolor extract of different methods of hypoglycemic effect in mice.docVIP

 PAGE \* MERGEFORMAT 2 Potentilla discolor extract of different methods of hypoglycemic effect in mice Potentilla discolor (Potentilla discolor Bunge) Science and Technology Commission for the Rosaceae Potentilla perennial herbs, roots, or dried whole plant can be used as medicine. Are distributed throughout the country, for our traditional Chinese medicine 〔 1〕 . In recent years, a popular treatment of diabetes with Potentilla discolor and achieved a certain effect. To understand the mechanism of hypoglycemic effect of Potentilla discolor, develop diabetes drugs or health products, this study was extracted from Potentilla discolor, according to the three parts of different polarity, using alloxan-induced diabetic mouse model of active screening, in order to identifying a strong hypoglycemic activity part. 1 Materials and methods 1  1 experimental animals Kunming mice (male) 100 (Kunming Institute of Zoology) (certificate number: Yunnan Laboratory Animal Permit No. 2002106 number). 1  2 Main reagents and instruments of alloxan (Sigma, USA); glucose detection kit (Shanghai Rongsheng Biotech Co., Ltd., batch number 20,030,101): Xiaoke Pill (Guangzhou Zhongyi Pharmaceutical Co., Ltd., batch number: E01895 ); 722-type grating spectrophotometer (Shanghai Analytical Instruments Factory). 1  3 Method 1  3  1 and the initial separation of the sample extracted using ethanol extraction, ethyl acetate extraction, D101 macroporous resin column chromatography, the I ~ Ⅲ Potentilla discolor is divided into parts, corresponding to D101 macroporous resin, water-eluted part, D101 part of the macroporous resin eluting methanol, ethyl acetate extract. 1  3  2 diabetes model of experimental animals 〔 2,3〕 experimental animal body weight (26 ± 2) g, fasted 16h, prepared to give alloxan modeling. Alloxan solution prepared immediately before use, the concentration of 12mg/ml. Manner by intraperitoneal injection administered dose 120mg / (kg * bw), a week late