Predicted SARS coronavirus spike protein S2 subunit of B-cell epitope peptide in E. coli and its antigenicity identification of.doc

 PAGE \* MERGEFORMAT 4 Predicted SARS coronavirus spike protein S2 subunit of B-cell epitope peptide in E. coli and its antigenicity identification of Authors: Feng Yu, Wan Min, Wang Xuan-Jun, Zhang because, in Yongli, Li-Ying Wang [Keywords:] SARS-CoV spike protein;, B cell epitope prediction;, partner 10 molecules;, S2 subunit Expression of predicted B cell epitope peptide in S2 subunit of SARS coronavirus spike protein in E.coli and identification of its mimic antigenicity [Abstract] AIM: To study the expression of predicted B cell epitope peptide in S2 subunit of SARS coronavirus spike protein in E.coli and its mimic antigenicity to S2 protein. METHODS: B cell epitopes in S2 subunit of SARS coronavirus spike protein was predicted using DNAStar software. The cDNA sequence encoding the B cell epitope peptide was constructed artificially by PCR and then cloned into the downstream of chaperone 10 gene in vector pET28a () to construct pET28chap10S2epi plasmid. The fusion protein, chap10S2epi, was expressed in E.coli BL21 (DE3) and identified by SDSPAGE and Western blot. The rabbit was immunized by purified Chap10S2epi for the preparation of antiserum, which was used to identify the mimic antigenicity of Chap10S2epi to S2 protein by ELISA. RESULTS: Chap10S2epi fusion protein was successfully constructed and expressed in E.coli. The antiserum from the animal immunized by Chap10S2epi recognized full length of SARS coronavirus S2 spike protein. CONCLUSION: The predicted B cell epitope peptide of SARS coronavirus S2 spike protein can induce the antigenicity of S2 protein, which provides some fundamental data for developing engineering vaccine against SARS coronavirus infection. [Keywords] SARS spike protein; B cell epitope prediction; chaperone 10; S2 subunit [Abstract] Objective: To study the predicted SARS-CoV spike protein S2 subunit of B-cell epitope peptide in E. coli and its simulation S2 protein antigenicity. Methods: The S2 subunit of DNAStar software f