Prescription freckle Tang on A375 human melanoma cells melanin synthesis.docVIP

 PAGE \* MERGEFORMAT 10 Prescription freckle Tang on A375 human melanoma cells melanin synthesis Author: Liu Min, Zhang Juan, Tao Rong, Su Yongmei, Yi Ru Di [Abstract] Objective To study the prescription freckle Tang on A375 human melanoma cell line cell proliferation, tyrosinase activity and melanin synthesis to explore the treatment of melasma prescription mechanism of action. Methods tetrazolium blue method (MTT) measured the impact of prescription on cell proliferation, were measured using enzymatic effect on tyrosinase activity, 475nm colorimetry prescription impact on the melanin content. The results prescription freckle Tang on cultured A375 human melanoma cells, inhibit cell proliferation, reduce tyrosinase activity and melanin content in the role. Conclusion This can only inhibit melanin synthesis of melanocytes, which may be the treatment of melasma one of the mechanisms. [Keywords:] prescription freckle soup; human melanoma cells; melanin synthesis; Melasma Abstract: ObjectiveTo study the effect of proved recipe Quban soup on cell proliferation, tyrosinase activity and the melanogenesis of A375 human melanoma cell and to explore the mechanism of them on treating chloasma. Methods MTT was used to study the proliferation of A375 human melanoma cell. Tyrosinase activity was measured by zymologic method. The melanin was detected by its absorption at 475nm. ResultsProved recipe Quban soup had the effects of inhibiting the proliferation, and decreasing tyrosinase activity and melanin content of A375 human melanoma cell in vitro. ConclusionThe recipe is effective in the treatment of chloasma by inhibiting the melanogenesis of A375 human melanoma cell. Keywords:: Proved recipe Quban soup; Human melanoma; Melanogenesis; Chloasma Melasma is a facial hair in the Department of pigmented skin disease, its pathogenesis mainly related to the liver, spleen, kidney related. Mentor Professor Di Yi Ru nourishing liver and kidney, nourishing Qi, promoting blood