Power for the ovariectomized rat hypothalamus neuroendocrine effects of signal transduction.docVIP

 PAGE \* MERGEFORMAT 17 Power for the ovariectomized rat hypothalamus neuroendocrine effects of signal transduction 【Abstract】 Objective: To compare the normal rats at different stages of sexual cycle, and electro-acupuncture treatment in ovariectomized rat hypothalamus medial preoptic area (medial preoptic area, MPOA) push-pull perfusion fluid of the main types of neural elements and content of information in order to observe physiological and pathological state, hypothalamic neuroendocrine similarities and differences in signal transduction pathways, preliminary study of electroacupuncture treatment of menopause syndrome neuro-endocrine signal transduction mechanisms. Methods: The rat brain three-dimensional positioning and nuclei push-pull perfusion technique to collect the normal sexual cycle of female rats at different stages of the hypothalamic MPOA push-pull perfusion fluid and electro-acupuncture in ovariectomized rats after the MPOA perfusate [referred to as acupuncture perfusion Liquid (acupuncture perfusate, AP)], respectively, the different micro-injected into the perfusion fluid in ovariectomized rats, MPOA, observe their respective ovariectomized rat vaginal cytology and serum estradiol (estradiol, E2) levels of impact. By radioimmunoassay and high performance liquid chromatography perfusate of each group in the gonadotropin-releasing hormone (gonadotropin  releasing hormone, GnRH), dopamine (dopamine, DA), γ  aminobutyric acid (gamma  aminobutyric acid, GABA ), glutamate (glutamic acid, Glu), aspartate (aspartic acid, Asp), and β  endorphin (β  endorphin, β  EP) content. Perfusate of each group in the type and content of these substances to conduct comparative analysis. Results: The normal sexual cycle of four different stages of perfusate in the DA, GABA, Glu and β  EP and other neurological content of active substance are different; MPOA of ovariectomized rats microinjection of normal sexual cycle of four phases of perfusion fluid,