Pro-apoptotic gene bax in bone giant cell tumor of the expression and significance of.doc

 PAGE \* MERGEFORMAT 8 Pro-apoptotic gene bax in bone giant cell tumor of the expression and significance of Keywords:: pro-apoptotic bax gene expression in giant cell tumor of bone Abstract Objective: To study the apoptosis promoting gene bax in bone giant cell tumor of the expression and Jaffe grade and prognosis. Methods: Rabbit anti-human Bax polyclonal antibody and immunohistochemistry SABC method. Results: 73 patients with giant cell tumor of bone, Bax-positive in 47 cases (64.4%); which Jaffe pathological grade Ⅰ 27 cases, positive in 22 cases (81.5%), Ⅱ grade 38 cases positive in 23 cases (60.5%) , Ⅲ grade 8 cases, 2 cases were positive (25.0%), P amp;lt;0.05. Prognosis in different groups in the positive rate of Bax was no significant difference between the 0.05). Conclusion: The pro-apoptotic gene in giant cell tumor of bone in different levels of expression may be involved in apoptosis of bone giant cell tumor of the regulation, and with the degree of tumor differentiation. Single Bax expression do not reflect the prognosis. Keywords:: apoptosis bax Giant cell tumor of bone Immunohistochemistry Is well known that abnormal regulation of apoptosis will lead to tumorigenesis. bax gene library is found in white blood cells, a new gene, its transcription product of Bax and bcl-2 protein can form a heterodimer, inhibit the bcl-2 function and promote apoptosis [1]. Giant cell tumor of bone is the most common primary bone tumor, one of Europe and the United States people are three times the incidence of [2,3]. The tumors ranged between benign and malignant, but the strong local invasive, some benign giant cell tumor of bone metastasis can occur [4]. Conventional surgical treatment of high recurrence rate. Therefore, regulation of apoptosis of bone giant cell tumor of the study on biological characteristics of understanding of their significance. In this paper, immunohistochemical method, Bax giant cell tumor of bone cell location and in different