Progress in proteomics technology and new.docVIP

 PAGE \* MERGEFORMAT 4 Progress in proteomics technology and new [Keywords:] proteomics multidimensional liquid chromatography mass spectrometry imaging technologies and labeling of affinity chromatography isotope chemistry ink blot protein chip 0 Introduction Proteomics is targeted proteomics research a new subject is the human genome project is completed the field of life sciences, one of the most active in recent years the rapid development of the corresponding methodology has also made great progress. Although the two-dimensional electrophoresis  mass spectrometry has been the core technology of proteomics, but in recent years a series of new technology, new ideas incorporated into the booming proteomics technology, which greatly contributed to this emerging discipline in the life sciences in various fields applications [1]. In this paper, proteomics research to do an overview of new technologies. An imaging mass spectrometry (Mass Spectrometry Imaging, MSI) technology MS imaging technique is the use of matrix-assisted laser desorption / ionization mass spectrometry to directly define the fresh frozen tissue sections of multi-peptide or protein, new technologies, also known as in-situ proteomics [2]. The basic process is to frozen tissue sections placed on a metal plate, coated substrate, UV pulsed laser excitation matrix of protein ionization measured quality of the nuclear ratio; the use of the organization access to thousands of points on the grating peak density value, resulting eventually in specific quality of mass spectrometry of molecular imaging. Usually this technique can be detected anywhere in the organization of more than 400 protein signal. MALDI-MS imaging of both MS equipment all the advantages of high sensitivity, but also with the simultaneous detection of the ability to multi-component mixtures, the basic need to consider chemical properties and molecular weight. MALDI MSI can be used for biological markers, such as peptides an